FIG 7.

Northern blot analysis of S. aureus HP1 loop mutant containing pEPSA-teg49 in the presence of 1% xylose. An S. aureus HP1 loop mutant harboring pEPSA5-teg49 or control empty pEPSA5 was grown in TSB containing 1% xylose at 37°C, and cells were harvested at late exponential phase (OD₆₅₀ of 1.1) (A, B) and at three different growth stages (OD₆₅₀s of 0.7, 1.1, and 1.7) (C,D). Purified total RNAs (10 μg/lane) were separated in a 1.5% agarose gel, transferred to H⁺-bond membranes, and then hybridized with radiolabeled DNA probes for teg49 (A), sarA (B), agrA (C), and aur (D).