TABLE 1.
Strains used in this study
| S. aureus strain | Genotype and/or characteristic | Source or reference |
|---|---|---|
| RN4220 | Mutagenized strain 8325-4 that accepts foreign DNA | 17 |
| SH1000 | Functional rsbU derivative of 8325-4 rsbU+ | 19 |
| Newman | Isolated from a human infection in 1952 | 41 |
| ALC6094 | Strain Newman transduced with IPTGb-inducible T7 polymerase; Tetr | 42 |
| ALC7201 | ALC6094 ΔcshA::Kanr | This study |
| ALC7252 | ALC7201 ΔcshA::cshA | This study |
| ALC7286 | SH1000 sarA deletion | This study |
| ALC7288 | SH1000 HP1 stem mutation of teg49 | This study |
| ALC7289 | SH1000 HP1 loop mutation of teg49 (ATTGCGC→CGCTATA)a | This study |
| ALC7290 | SH1000 HP2 loop mutation of teg49 (GTCGATT→TATCGGC)a | This study |
| ALC7291 | SH1000 HP2 stem mutation of teg49 | This study |
| ALC7869 | Tn551::sigB in ALC6094 | This study |
| ALC7870 | ALC7869 containing pSK236::sigB | This study |
| ALC7909 | ALC7289 containing empty vector pEPSA5 | This study |
| ALC7911 | ALC7289 containing pEPSA5::teg49 | This study |
a
Nucleotides replaced in chromosomal DNA.
b
IPTG, isopropyl-β-d-thiogalactopyranoside.