FIG 1.
Growth of M. tuberculosis strains in liquid and solid media. (A) Wild-type M. tuberculosis H37Rv, ΔcuvAMt, and complemented ΔcuvA (ΔcuvAMt/C) strains were grown in 7H9-ADC-Tw liquid medium with 0.2% glucose, and the OD600 was measured daily. (B) Growth of the same strains on agar plates containing MM plus 0.2% glucose or MM plus 0.01% cholesterol. Serial 10-fold dilutions were spotted and incubated at 37°C, and photographs were taken after 1 month of incubation. In both panels, data shown are from one experiment that was repeated at least twice with similar results. Glc, 0.2% glucose; Chol., 0.01% cholesterol.