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Colocalization of CuvA and nascent peptidoglycan synthesis in M. smegmatis. Wild-type M. smegmatis mc2155 cells expressing GFP alone or GFP fused to CuvAMt were grown in MM-ADC-TW containing 0.2% glucose or MM containing 0.01% cholesterol. Vancomycin labeled with Alexa 568 was used to bind to newly synthesized peptidoglycan. Fluorescence and DIC microscopy images were obtained to determine the localization of CuvAMt-GFP and nascent peptidoglycan synthesis. Bars = 1 μm.
