FIG 3.

Transcription of antimicrobial peptide genes Diptericin and Defensin in the five Drosophila melanogaster laboratory strains Oregon, w1118, Canton-S, Cinn. Brown, and YW after infection with the Gram-negative bacteria Escherichia coli (strain DH5a) (A) and Photorhabdus luminescens (strain TT01) (B) and the Gram-positive bacteria Micrococcus luteus (strain CIP A270) (C) and Enterococcus faecalis (strain ATCC 19433) (D). Flies (n = 20 per infection treatment) were injected with PBS buffer containing approximately 100 to 150 bacterial cells. Gene transcription was estimated at 0, 3, 6, 12, 24, and 48 h after infection. Diptericin and Defensin mRNA levels are shown as the relative abundance of transcripts normalized to RpL32 and are expressed as a ratio compared to flies injected with PBS only (negative controls). Values represent the means from three biological replicates, and error bars represent standard deviations. Asterisks show a value that is significantly different (one-way ANOVA with a Tukey post hoc test for multiple comparisons). **, P < 0.01; *, P < 0.05; n.s., nonsignificant differences.