FIG 4.

Numbers of circulating hemocytes recovered from the five Drosophila melanogaster laboratory strains Oregon, w1118, Canton-S, Cinn. Brown, and YW after injection with PBS buffer (control treatment) (A), the Gram-negative bacteria Escherichia coli (strain DH5a) (B) and Photorhabdus luminescens (strain TT01) (C), and the Gram-positive bacteria Micrococcus luteus (strain CIP A270) (D) and Enterococcus faecalis (strain ATCC 19433) (E). Flies (n = 20 per condition) were injected with PBS buffer containing approximately 100 to 150 bacterial cells. Hemocyte counts were determined per microliter of hemolymph at 0, 3, 6, 12, 24, and 48 h after fly injection. Bars represent the means from three separate experiments, and error bars represent the standard deviations. The asterisk shows a value that is significantly different from the other treatments (one-way ANOVA with a Tukey post hoc test for multiple comparisons). *, P < 0.05; n.s., nonsignificant differences between treatments.