FIG 8.

Survival results after injection of the Drosophila melanogaster laboratory strains w1118 and YW and the mutant strains for NOS, NOS²⁴²⁸³, and NOS⁵³¹⁵⁶ with PBS buffer (negative controls) (A), the Gram-negative bacteria Escherichia coli (strain DH5a) (B) and Photorhabdus luminescens (strain TT01) (C), and the Gram-positive bacteria Micrococcus luteus (strain CIP A270) (D) and Enterococcus faecalis (strain ATCC 19433) (E). Each replicate consisted of ten 4- to 6-day-old flies injected with PBS buffer containing 100 to 150 bacterial cells. The survival of flies was monitored every 24 h and up to 144 h after infection, and data represent the percent survival of infected flies. The averages from three separate experiments are shown. Survival curves were compared by using a log-rank (Mantel-Cox) test.