FIG 6.

Regulation of bb0238. (A and B) qRT-PCR (A) and immunoblot analysis (B) of bb0238 expression in Bb297 cultivated to mid-exponential growth phase at 23°C, pH 7.5, or grown to the late exponential growth phase at 37°C, pH 6.8. (C and D) qRT-PCR (C) and immunoblot analysis (D) of bb0238 expression in BbiEbfC without IPTG or induced with 1 mM IPTG for 48 h. Detection of OspC protein and ospC transcript was used as a positive control to confirm rpoS activation. Detection of DbpA protein and ebfC transcript was included as a positive control to confirm ebfC expression. Relevant protein molecular masses (lane MW) are shown on the left in kDa. The immunoblot results are representative of two independent experiments. The qRT-PCR results are based on data from three biological replicates. The error bars represent SEM. Statistical significance was determined using an unpaired Student t test. The asterisks indicate a statistically significant difference between the respective conditions (P < 0.05).