FIG 9.
Characterization of BB0238 TPR point mutants. (A) Consensus sequence of the TPR motif predicted from sequence alignment of known TPR-containing proteins (13). The horizontal bars below the alignment indicate helices A and B. (B) BB0238 predicted TPR region (residues 40 to 73), with consensus residues in boldface and conserved residues underlined. The TPR mutations engineered in BB0238 are shown below the parental sequence, with the targeted residue(s) in boldface and the mutated residue above. (C) Immunoblot analysis of BB0238 expression in Bb297, BbΔbb0238, Bbbb0238C, Bbbb0238Y43A, Bbbb0238G47V, Bbbb0238Y50A, Bbbb0238A66G, and Bbbb0238P71A assessing protein expression of native and mutated BB0238. Detection of FlaB was included as a loading control. The relevant molecular weight markers (lane MW) are noted on the left. The immunoblot results are representative data from two independent experiments.