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. 2014 Oct;82(10):4222–4232. doi: 10.1128/IAI.02336-14

FIG 2.

FIG 2

PCR screening of the strains used in this study. For htrA PCR, the primers oDB0123 and oDB0124 generated a PCR product of approximately 2,200 bp in the htrA mutant (ΔhtrA, DB0002) and a PCR product of 150 bp in the wild type (WKS1241), wild type/pRPF185 (DB0051), complemented htrA mutant (ΔhtrA/htrA+, DB0047), and ΔhtrA/pRPF185 (DB0052). For pRPF185 PCR, the primers oWKS-1177 and NF794 generated a PCR product of approximately 1,800 bp in the DB0051 and DB0052 strains. For htrA-ClosTron PCR, the primers oDB0123 and EBSuni generated a PCR product of approximately 280 bp in the DB0002, DB0047, and DB0052 strains. For Ptet-htrA PCR, the primers oDB0123 and NF794 generated a PCR product of approximately 1,200 bp in DB0047.