FIG 7.

Agr activation by sAIP does not promote hemolytic activity in concentrated cultures in the absence of a functional electron transport chain. Wild-type SH1000 and a hemB::Tn mutant were concentrated to equivalent bacterial densities and cultured in TSB in the absence or presence of HQNO and the absence (open bars) or presence (filled bars) of 10 μM sAIP. The data represent the mean averages of 4 experiments performed in triplicate, and the error bars represent the standard deviations of the mean. None of the values obtained from cultures containing sAIP were significantly different from those without sAIP at identical dilutions.