TABLE 1.
Bacterial strains and plasmids used in this study
| Bacterial strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| S. aureus | ||
| SH1000 | Functional rsbU+ strain repaired for rsbU from parental laboratory strain NCTC8325-4 | 42 |
| SH1001 (agr mutant) | SH1000 agr::tet | 42 |
| SH1000 hemB::Tn | SH1000 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph | This study |
| SH1001 hemB::Tn | SH1001 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph | This study |
| SH1000-P3 | pCL55agr IR P3-GFP integrated into the geh locus of SH1000; reports P3 activity | 44 |
| SH1000-P2 | pCL55agr IR P2-GFP integrated into the geh locus of SH1000; reports P2 activity | 44 |
| SH1001-P3 | pCL55agr IR P3-GFP integrated into the geh locus of SH1001; reports P3 activity | 44 |
| SH1001-P2 | pCL55agr IR P2-GFP integrated into the geh locus of SH1001; reports P2 activity | 44 |
| SH1000 hemB::Tn-P3 | SH1000-P3 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph; reports P3 activity | This study |
| SH1000 hemB::Tn-P2 | SH1000-P2 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph; reports P2 activity | This study |
| SH1001 hemB::Tn-P3 | SH1001-P3 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph; reports P3 activity | This study |
| SH1001 hemB::Tn-P2 | SH1001-P2 transduced with DNA from a USA300 hemB::Tn mutant; Ermr; heme auxotroph; reports P3 activity | This study |
| USA300 LAC | CA-MRSA strain of the USA300 lineage | 43 |
| USA300 hemB | USA300 in which hemB has been deleted | This study |
| USA300 menD | USA300 in which menD has been deleted | This study |
| USA300 hemB-P3 | USA300 hemB; reports P3 activity | This study |
| USA300 menD-P3 | USA300 menD; reports P3 activity | This study |
| E. coli | ||
| DC10B | Δdcm in DH10B (ΔhsdRMS endA1 recA1) dam methylation only; high-efficiency cloning strain that bypasses the restriction modification system of S. aureus | 46 |
| Plasmids | ||
| pCL55agr IR P3-GFP | pCL55 Ar Cmr containing the agr P2-P3 intergenic region with gfp expression under the control of P3 | 44 |
| pCL55agr IR P2-GFP | pCL55 Ar Cmr containing the agr P2-P3 intergenic region with gfp expression under the control of P2 | 44 |