FIG 6.

Effects of aspirin upon NO production in T. cruzi-infected H9C2 cells. Cells were treated for 1 h with ASA (0.625, 1.25, and 2.5 mM) (a) or celecoxib (0.625, 1.25 and 2.5 mM) (b) and exposed to T. cruzi (Y strain). After treatment with NSAIDs, cells interacted with a 5:1 trypomastigote:cell ratio for 24 h, after which they were washed and cultured at 37°C in 5% CO₂ during 24 h. NO levels in supernatants were measured by high-sensitivity chemiluminescence. Results are the means ± standard errors of the means for duplicate determinations and are representative of two independent experiments. *, P < 0.05; **, P < 0.01 (compared to the untreated infected cell culture [Tc]).