FIG 8.

Aspirin in combination with 10 μM forskolin increased TGF-β production by T. cruzi-infected H9C2 cells. Cells were treated for 1 h with ASA (2.5 mM). After treatment, cells were washed and incubated with 10 μM forskolin for 20 min. After treatment, cells were washed again and allowed to interact with a 5:1 trypomastigote:cell ratio for 24 h at 37°C. TGF-β levels in supernatants were measured with a specific enzyme-linked immunosorbent assay. Results are means ± standard errors of the means for duplicate determinations and are representative of two independent experiments. *, P < 0.05 (compared to infected cells in cell culture medium, ASA, or forskolin alone).