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. 2014 Oct;58(10):6324–6327. doi: 10.1128/AAC.03354-14

TABLE 2.

Kinetic parameters of AAC(6′)-Iak and AAC(6′)-Iz enzymes^a

Aminoglycoside^b	AAC(6′)-Iak			AAC(6′)-Iz
Aminoglycoside^b	K_m (μM)^c	k_cat (s⁻¹)^c	k_cat/K_m (μM⁻¹ s⁻¹)	K_m (μM)^c	k_cat (s⁻¹)^c	k_cat/K_m (μM⁻¹ s⁻¹)
ABK	36 ± 13	0.57 ± 0.10	0.016	17 ± 6	0.57 ± 0.06	0.036
AMK	32 ± 17	0.11 ± 0.03	0.004	24 ± 5	0.26 ± 0.02	0.011
DIB	24 ± 3	0.25 ± 0.04	0.010	31 ± 5	0.46 ± 0.05	0.015
ISP	44 ± 9	0.05 ± 0.01	0.001	49 ± 8	0.11 ± 0.01	0.002
KAN	30 ± 7	0.04 ± 0.01	0.001	44 ± 9	0.05 ± 0.01	0.001
NEO	10 ± 2	0.32 ± 0.01	0.034	13 ± 2	0.29 ± 0.01	0.023
NET	70 ± 6	0.30 ± 0.02	0.004	25 ± 8	0.34 ± 0.05	0.013
SIS	4 ± 1	0.16 ± 0.01	0.038	7 ± 1	0.20 ± 0.03	0.028
TOB	16 ± 4	0.08 ± 0.01	0.006	12 ± 2	0.25 ± 0.03	0.021

^a

The proteins were initially modified by a His tag, which was removed after purification.

^b

ABK, arbekacin; AMK, amikacin; DIB, dibekacin; ISP, isepamicin; KAN, kanamycin; NEO, neomycin; NET, netilmicin; SIS, sisomicin; TOB, tobramycin.

^c

K_m and k_cat values represent the means of results from 3 independent experiments ± standard deviations.