TABLE 1.
Comparison of IC50 values of antimalarials against P. falciparum isolates and the W2 reference clone
| Test plate compounds | GM IC50, nM |
Isolate/W2 GM IC50 ratio | P valuec | |
|---|---|---|---|---|
| Isolates (range)a | W2 (95% CI)b | |||
| Blinded test compounds | ||||
| Artemisone | 2.4 (0.02–117.7) | 1.9 (1.5–2.4) | 1.3 | 0.004 |
| DHA internal control | 8.0 (1.5–38.1) | 5.0 (4.2–5.9) | 1.6 | <0.001 |
| AS | 8.5 (0.5–52.7) | 6.4 (5.0–8.1) | 1.3 | 0.019 |
| DHA | 11.2 (0.04–109.4) | 5.2 (3.8–7.1) | 2.2 | <0.001 |
| Artemiside | 15.3 (0.8–458.7) | 11.2 (8.7–14.3) | 1.4 | 0.022 |
| OZ277 | 31.3 (0.02–320.7) | 38.5 (30.2–49.1) | 0.8 | 0.726 |
| OZ78 | 755.7 (65.7–7,854) | 406.7 (319.8–517.2) | 1.9 | 0.003 |
| Standard drug panel | ||||
| AS | 5.8 (0.6–24.6) | 4.7 (4.4–5.1) | 1.2 | <0.001 |
| LUM | 7.4 (0.4–40.1) | 6.0 (4.9–7.3) | 1.2 | 0.002 |
| DHA | 9.0 (0.8–30.2) | 4.5 (4.1–4.9) | 2.0 | <0.001 |
| MQ | 58.3 (0.7–205.5) | 20.6 (18.2–23.2) | 2.8 | <0.001 |
| QN | 146.1 (2.2–439.2) | 149.0 (135.9–163.4) | 1.0 | 0.878 |
| CQ | 147.8 (4.7–786.0) | 258.2 (237.7–280.5) | 0.6 | <0.001 |
a
GM IC50s were determined for a total of 200 isolates.
b
GM IC50s against W2 in the blinded test compound panel were determined from n = 51, 52, 52, 48, 52, 51, and 48 independent experiments for artemisone, DHA internal control, AS, artemiside, OZ277, and OZ78, respectively. GM IC50s in the standard drug panel against W2 were determined from 123 independent experiments.
c
P values (Mann-Whitney U test) in bold indicate a significant difference between IC50s for each evaluated antimalarial against isolates and W2.