TABLE 1.

Bacterial strains and plasmids used in this study

Plasmid or strain	Description	Reference/source
Plasmids
pMAD	E. coli-S. aureus shuttle vector containing a thermosensitive origin of replication, bgaB	17
pMADΔtrx	pMAD containing up- and downstream regions of sacol1794	This study
pMADΔmmpL	pMAD containing up- and downstream regions of sacol2566	This study
pCN40	E. coli-S. aureus shuttle vector	33
pCNtrx	pCN40 harboring sacol1794 gene and its promoter	This study
pCNtrxP70Q	pCNtrx harboring mutated sacol1794 gene that resulted in P70Q substitution in its product	This study
pCNmmpL	pCN40 harboring sacol2566 gene and its promoter	This study
pCNmmpLT172I	pCNmmpL harboring mutated sacol2566 gene resulted in T172I substitution in its product	This study
S. aureus strains
COL	Hospital-acquired methicillin-resistant S. aureus parent strain	NARSA
COLR	COL expressing resistance to oxadiazole antibiotics	This study
COLI	COL expressing intermediate resistance to oxadiazole antibiotics	This study
COLΔtrx	COL lacking sacol1794	This study
COLΔmmpL	COL lacking sacol2556	This study
RN4220	Restriction-deficient derivative of NCTC 8325-4	34
RN9594	RN4220 containing pCN40	33
RN4220Δtrx	RN4220 lacking sacol1794	This study
RN4220ΔtrxΩ	RN4220Δtrx strain containing pCN40 (vector-only control)	This study
RN4220ΔtrxΩtrx	RN4220Δtrx strain complemented by pCNtrx	This study
RN4220ΔtrxΩtrxP70Q	RN4220Δtrx strain complemented by pCNtrxP70Q	This study
RN4220ΔmmpL	RN4220 lacking sacol2556	This study
RN4220ΔmmpLΩ	RN4220ΔmmpL strain containing pCN40 (vector only control)	This study
RN4220ΔmmpLΩmmpL	RN4220ΔmmpL strain complemented by pCNmmpL	This study
RN4220ΔmmpLΩmmpLT172I	RN4220ΔmmpL strain complemented by pCNmmpLT172I	This study
E. coli strain
DH5α	supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1	35