TABLE 2.
Primers used in this study for deletion of sacol1794 and sacol2566 and complementation
| Usage and name | Sequence (5′ to 3′)a | Restriction site |
|---|---|---|
| Deletion mutant | ||
| D1794UF | CCCGGATCCCACGCCATTCACACAGTTATCT | BamHI |
| D1794UR | CCCCTCGAGTTTCATTTTTATGTCTCCTTAATTAATGAT | XhoI |
| D1794DF | CCCCTCGAGGTGTAATTTAGACTAGAGAAAAACGGGGTA | XhoI |
| D1794DR | CCCCATGGCTAAACGCTGAATCACGTTTGGAT | NcoI |
| D2566UF | CCCGGATCCGGTAACGCTCATGAGTTTCTCATCTAC | BamHI |
| D2566UR | CCCCTCGAGTGCCAAAGTATATTGCCTCCTTTTAA | XhoI |
| D2566DF | CCCCTCGAGTAAAATAACATGTACATGCCTCCGC | XhoI |
| D2566DR | CCCCGGGGCGGTTGATACCCTTCCCCTA | SmaI |
| Complementation | ||
| Trx-F | CCCCCCGGATCCTCGGGTAATAACAACTATTATCTCTAAATAGTTATATATAATC | BamHI |
| Trx-R | CCGGCGCCTTACACGTATTGAGCTAAAAATGCATCTATC | NarI |
| MmpL-F | GGGGCATGCTTGTAGCAAGCTACTCGACAATGC | SphI |
| MmpL-R | GGGGGTACCTTATGATTTATTTCGTAGATTTTTTTCTAACAATTG | KpnI |
a
Restriction sites are underlined.