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Effects of the time of RDS1759 addition on HIV-1 replication. HIV-1-infected MT4 cells were treated with 10 μM RDS1759, 200 nM RAL, or 100 nM EFV at different time points. The x axis shows the time (h) of compound addition. Samples were collected at 24 and 48 h, and integrated viral DNA was measured. All results were quantified by qPCR and normalized to values for infected untreated cells. The experiment was performed three times (error bars represent standard deviations).
