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Selection of ODC-overexpressing cells from the library under DFMO pressure. (A) Cumulative growth curves for the trypanosome library in the presence of 60 μM DFMO. Samples of cells were collected at day 12 (arrow). (B) Library inserts amplified with primers 1 and 5 (Fig. 1), using genomic DNA as the template. Star, PCR product encoding ODC. The band indicated by the arrowhead did not contain the ODC gene.
