FIG 4.

Selection of NMT-overexpressing cells under DDD85646 pressure. (A and B) Cumulative growth curves for the trypanosome library in the presence of a range of concentrations (5, 6, 6.5, 8.5, and 12.5 nM) of DDD85646. Arrows, times when samples of cells were collected. In panel B, the cells were below the detection limit from day 2 to day 6. To simplify the experiment, no culture without tetracycline was included. (C) Library inserts amplified with primers 1 and 5 (Fig. 1), using genomic DNA as the template. Stars, bands encoding N-myristoyltransferase. The band indicated by the arrowhead did not encode N-myristoyltransferase.