FIG 5.

R4.0 acts as a CCR5 antagonist. (A to D) Immunofluorescence of CHO CD4 CCR5 cells labeled with the anti-CCR5 MAb 3A9 (A) or preincubated with 100 nM CCL5 (B), 100 nM MVC (C), or 100 nM R4.0 (D) and labeled with 3A9. (E) CHO CD4 CCR5 cell surface CCR5 measured by flow cytometry was set at 100% for cells treated with 3A9. The CCR5 cell surface expression of cells preincubated with CCL5, MVC, or R4.0 is shown as a mean percentage of the expression in control cells ± SD and is representative of two independent experiments.