FIG 9.

Effect of resveratrol on the arginase activity of murine macrophages. (A) Macrophages (10⁶) were stimulated with 20 ng/ml IL-4 or left unstimulated and then treated with 100 μM resveratrol (Rv) for 48 h. (B) Macrophages (10⁶) were stimulated with 20 ng/ml IL-4 or left unstimulated and then treated with 1 μM AMB. (C) Macrophages (10⁶) were infected with Leishmania (L) promastigotes at a 5:1 ratio for 24 h, stimulated with 20 ng/ml IL-4 or left unstimulated, and then treated with 100 μM Rv or 0.5 μM amphotericin B (AMB) or left untreated for 48 h. (D) Recovery of infectivity by spermidine in Rv-treated macrophages. Macrophages (10⁶) were infected with promastigotes at a 10:1 ratio for 24 h and subsequently treated with 100 μM Rv, 100 μM spermidine, or both Rv and spermidine for 24 h. The results represent the means ± SEM from three (A and B) or two (C) experiments performed in triplicate. *, P < 0.05; **, P < 0.001; ***, P < 0.0001.