FIG 5.

B. thailandensis fails to form actin tails inside IFN-γ-treated macrophages. RAW cells were infected with B. thailandensis and not treated (A) or treated with ceftazidime (10 μg/ml) (B), IFN-γ (10 ng/ml) (C), or ceftazidime and IFN-γ (D) for 12 h. Macrophages were then fixed, permeabilized, and stained with phalloidin to identify host cell actin (green), DAPI to stain nuclei (blue), and anti-Burkholderia serum followed by secondary antibody conjugated to Cy3 to identify B. thailandensis (red). Images were captured under ×40 magnification. Actin tails are seen as bright green protrusions from the red bacteria in panels A and B. Images are representative of data from three independent experiments.