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EptC active-site mutant motility assays. Parent strain (81-176), eptC null mutant (ΔeptC), complemented ΔeptC strain (ΔeptC/eptC +) and strains carrying eptC active-site mutant alleles (T266A, T266S, N308A, S309A, H358A and H440A) were stab-inoculated into semisolid MH motility medium and incubated under microaerobic conditions. To assess relative motility, bacterial growth diameters were visually compared 24 h post-inoculation.
