|
Escherichia coli
|
| XL1-Blue |
General cloning strain, recA1 endA1 gyrA96thi-1 hsdR17
|
Stratagene |
| BL21(DE3) |
Protein-expression strain, F−
ompT gal dcm lon hsdSB(rB
− mB
−) λ(DE3) |
Novagen |
|
Campylobacter jejuni
|
| 81-176 |
Serotype HS: 23, 26 |
Cullen & Trent (2010 ▶) |
| 81-176 A1 |
81-176 Δcj0256 clone1, CamR
|
Cullen & Trent (2010 ▶) |
| 81-176 B |
Complement strain, 81-176 Δcj0256 ΔastA::cj0256, CamR, KanR
|
Cullen & Trent (2010 ▶) |
| 81-176 eptCT266A
|
81-176 Δcj0256 ΔastA::cj0256
T266A, CamR, KanR
|
This study |
| 81-176 eptCT266S
|
81-176 Δcj0256 ΔastA::cj0256
T266S, CamR, KanR
|
This study |
| 81-176 eptCN308A
|
81-176 Δcj0256 ΔastA::cj0256
N308, CamR, KanR
|
This study |
| 81-176 eptCS309A
|
81-176 Δcj0256 ΔastA::cj0256
S308A, CamR, KanR
|
This study |
| 81-176 eptCH358A
|
81-176 Δcj0256 ΔastA::cj0256
H358A, CamR, KanR
|
This study |
| 81-176 eptCH440A
|
81-176 Δcj0256 ΔastA::cj0256
H4440A, CamR, KanR
|
This study |
| Plasmids |
| pET-28b(+) |
Expression vector containing a T7 promoter, AmpR
|
Novagen |
| pETcEptC |
pET-28b(+)-derived expression plasmid used to express cEptC, AmpR
|
This study |
| pAtsAKO::cj0256:KanR
|
pGEM-atsAKO:KanR with cj0256 inserted |
Cullen & Trent (2010 ▶) |
| pETcEptCT266S
|
T266S mutant derivative of pETcEptC, AmpR
|
This study |
| pAstAEptCT266A
|
T266A mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
| pAstAEptCT266S
|
T266S mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
| pAstAEptCN308A
|
N308A mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
| pAstAEptCS309A
|
S309A mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
| pAstAEptCH358A
|
H358A mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
| pAstAEptCH4440A
|
H440A mutant derivative of pAtsAKO::cj0256:KanR
|
This study |
