Table 2. Primers used in this study.

Underlined regions indicate introduced mutations.

1. F-VPIPEcEptC	5′-ACCCCCGCCTCCCCTGGATTTGCCTTAAGTTTAGGATTTAA-3′
2. R-VPIPEcEptC	5′-AGGAGATATACCATGTTTAAAACTATAGCTAATGATGCTTATA-3′
3. F-IPIPEcEptC	5′-GGGGAGGCGGGGGTGGACACCACCACCACCACCACCACCACTGAGAT-3′
4. R-IPIPEcEptC	5′-CATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATT-3′
5. F-EptCT266S	5′-ATTTTTCATCATGTGGAACAGCAAGCGCGGTAAGTTTGCCTTGTATGTTT-3′
6. R-EptCT266S	5′-AAACATACAAGGCAAACTTACCGCGCTTGCTGTTCCACATGATGAAAAAT-3′
7. F-EptCT266A	5′-ATTTTTCATCATGTGGAACAGCAGCGGCGGTAAGTTTGCCTTGTATGTTT-3′
8. R-EptCT266A	5′-AAACATACAAGGCAAACTTACCGCCGCTGCTGTTCCACATGATGAAAAAT-3′
9. F-EptCN308A	5′-GCATGGTTTGATAATGCATCTGGTGGTTGTAAA-3′
10. R-EptCN308A	5′-TTTACAACCACCAGATGCATTATCAAACCATGC-3′
11. F-EptCS309A	5′-TGGTTTGATAATAATGCAGGTGGTTGTAAAGGG-3′
12. R-EptCS309A	5′-CCCTTTACAACCACCTGCATTATTATCAAACCA-3′
13. F-EptCH358A	5′-CATTTGCAAGGTTCTGCAGGGCCAACTTATTAT-3′
14. R-EptCH358A	5′-ATAATAAGTTGGCCCTCGAGAACCTTGCAAATG-3′
15. F-EptCH440A	5′-AATGGTATTTATCTTGCAGGTATGCCTTATGCT-3′
16. R-EptCH440A	5′-AGCATAAGGCATACCTCGAAGATAAATACCATT-3′