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. 2014 Oct 7;5:225. doi: 10.3389/fphar.2014.00225

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Copyright © 2014 Cooper, Kliwinski, Perkinson, Ragwan, Mabus, Powers, Dorai, Giles-Komar and Hornby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface plasmon resonance (SPR) analysis using the ProteOn XPR36 in co-injection mode IgG1 variants, (A) wild-type (WT); (B) N434A or (C) N434Y were diluted 1:5 in a concentration series from 1000 to 1.6 nM, injected over rat FcRn for 1 min in PBS-TE (pH 6.0) and followed immediately by a co-injection of PBS-TE (pH 7.4) without mAb for 3 min.