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| Method | Hanging drop |
| Plate type | EasyXtal 15-Well Tool |
| Temperature (C) | 18 |
| Protein concentration (mgml1) | 8 |
| Buffer composition of protein and DNA solution | 40mM HEPES pH 7.5, 100mM NaCl, 5mM -mercaptoethanol |
| Volume and ratio of drop | 1:1 (1l:1l) |
| Volume of reservoir (l) | 500 |
| DNA element† (DC5_T/A) | 5-TTTGTTGCTCACCTACCATGGACAAT-3 |
| 3-AACAACGAGTGGATGGTACCTGTTAA-5 |
The DC5 core recognition site is underlined. Overhangs in the DNA element are italicized.
