Table 2. Crystallization.
| Method | Hanging-drop vapour diffusion |
| Plate type | 24-well NeXtal crystallization plate |
| Temperature (K) | 287 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 25mM TrisHCl pH 7.5, 30mM NaCl, 10%(v/v) glycerol |
| Composition of reservoir solution | 0.1M sodium citrate/citric acid pH 4.5, 19.8% PEG 3350 |
| Volume and ratio of drop | 1.8l (0.9l protein solution:0.9l reservoir solution) |
| Volume of reservoir (l) | 1000 |