Table 1. Macromolecule-production information.

Source organism	M. smegmatis MC2 155
DNA source	The genome of M. smegmatis
Forward primer†	GGAATTCCATATGGTGACGCGCGCAGGTCTTTCAC
Reverse primer‡	CCGGAATTCTCATGCCCGCGCGGTGGCGAG
Cloning vector	pET-28a
Expression vector	pET-28a:MsbioF
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLVPRGSHMMTRAGLSPLAWLADIEQRRRAEGLRRELRVRPPVAAELDLASNDYLGLSQHPDVLDGGVEALRTWGGGAGGSRLVTGNTELHEAFEHQLASFLGAESALVFSSGYTANLGALVALSGPGSLIVSDALSHASLVDACRLSRARVVVSPHRDVDAVDAALAARTEERAVVVTESVFSADGDLAPLRDLHAVCRRHGALLLVDEAHGLGVRGTRGQGLLHEVGLAGAPDIVMTTTLSKALGSQGGAVLGPEAVRAHLIDTARSFIFDTGLAPAAVGAASAALRVLDAEPQRARAVLDRAAELATIAGVTEAPVSAVVSVILGDPEIAVGAAAACLDRGVRVGCFRPPTVPAGTSRLRLAARASLTDDEMALARQVLTDVLATARA

^†The NdeI site is underlined.

^‡The EcoRI site is underlined.