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. 2014 Sep 25;70(Pt 10):1376–1379. doi: 10.1107/S2053230X14018408

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 96-well CrystalQuick Plus
Temperature (K) 293
Protein concentration (mgml1) 19
Buffer composition of protein solution 4mM TrisHCl pH 8.0
Composition of reservoir solution 0.1M TrisHCl pH 9.0, 10%(w/v) PEG 8000
Volume and ratio of drop 2.4l, 1:1
Volume of reservoir (l) 100