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. 2014 Sep 25;70(Pt 10):1402–1405. doi: 10.1107/S2053230X14018743

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 48-well sitting-drop plate
Temperature (K) 289
Protein concentration (mgml1) 20
Buffer composition of protein solution 500mM KCl, 20mM Tris pH 8.3
Composition of reservoir solution 0.2M sodium formate, 20%(w/v) polyethylene glycol 3350
Volume and ratio of drop 1l protein solution:1l precipitant solution
Volume of reservoir (l) 150