| Method |
Hanging-drop vapour diffusion |
Hanging-drop vapour diffusion |
| Plate type |
24-well VDX plate (Hampton Research) |
24-well VDX plate (Hampton Research) |
| Temperature (K) |
293 |
293 |
| Protein concentration (mgml1) |
10 |
10 |
| Buffer composition of protein solution |
20mM TrisHCl pH 8.0, 150mM NaCl, 5mM DMAPP, 2mM MgSO4
|
20mM TrisHCl pH 8.0, 150mM NaCl |
| Composition of reservoir solution |
100mM HEPESNaOH pH 7.0, 24% PEG 2000 |
100mM TrisHCl pH 7.0, 1M sodium citrate tribasic, 200mM NaCl |
| Volume and ratio of drop |
2l, 1:1 protein:reservoir solution |
2l, 1:1 protein:reservoir solution |
| Volume of reservoir (l) |
500 |
500 |
