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SDS–PAGE analysis of recombinant LgRec1 expression. Lane M, protein molecular-weight markers (Page Ruler, Thermo Scientific); lanes 1 and 2, extract from BL21 Star (DE3) pLysS without and with IPTG induction, respectively; lane 3, purified LgRec1 from Ni Sepharose (GE Healthcare). The numbers on the left correspond to the molecular masses in kDa of molecular-weight marker proteins in lane M.
