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| Method | Hanging-drop vapour diffusion |
| Plate type | Linbro 24-well tissue-culture plate |
| Temperature (C) | 18 |
| Protein concentration (mgml1) | 15 |
| Buffer composition of protein solution | Phosphate-buffered saline pH 7.0 |
| Composition of reservoir solution | 0.1M TrisHCl pH 8.2, 22% polyethylene glycol 10000 |
| Volume ratio of drop | 2:2 |
| Volume of reservoir (ml) | 0.5 |
