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. 2014 Sep 25;70(Pt 10):1424–1427. doi: 10.1107/S2053230X14019384

Table 1. Macromolecule-production information.

Source organism A. vinosum DSM 180T
DNA source Genomic
Forward primer CCACAAAGAAACATATGCGCGGTG
Reverse primer CCGACGGGTGATATCGCGTCG
Cloning vector pPR-IBA 1
Expression vector pPR-IBA 1
Expression host E. coli BL21 (DE3)
Complete amino-acid sequence of construct produced MEPPTVALTVPAAALLPDGALGESIVRGRRYLSDTPAQLPDFVGNGLACRHCHPGRDGEVGTEANAAPFVGVVGRFPQYSARHGRLITLEQRIGDCFERSLNGRALALDHPALIDMLAYMSWLSQGVPVGAVVAGHGIPTLTLEREPDGVHGEALYQARCLACHGADGSGTLDADGRYLFPPLWGPRSFNTGAGMNRQATAAGFIKHKMPLGADDSLSDEEAWDVAGFVLTHPRPLFQEPTGDAWSHPQFEK