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| Method | Vapour diffusion (sitting-drop) |
| Plate type | MRC Maxi 48-well (Swissci) |
| Temperature (C) | 20 |
| Protein concentration (mgml1) | 8 |
| Buffer composition of protein solution | 20mM Bis-TrisHCl pH 6.5 |
| Composition of reservoir solution | 23.5%(w/v) PEG 3350, 0.2M (NH4)2SO4, 0.1M Bis-Tris pH 6.28, 0.1M NaI |
| Volume and ratio of drop | 3l (1.2l protein + 1.5l precipitant + 0.3l additive) |
| Volume of reservoir (l) | 120 |
