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. 2014 Sep 25;70(Pt 10):1431–1433. doi: 10.1107/S2053230X14019712

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type Corning 3556
Temperature (K) 295
Protein concentration (mgml1) 4.2
Buffer composition of protein solution 10mM TrisHCl pH 7.5
Composition of reservoir solution 50mM MES pH 6.0, 3% PEG 4000, 15% 2-propanol, 50mM MgCl2
Volume and ratio of drop 750nl, 2:1 protein:well solution
Volume of reservoir (l) 80