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. 2014 Oct 7;8:315. doi: 10.3389/fncel.2014.00315

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Copyright © 2014 Xie, Choudhury, Wang, Park, Liu, Yuan, Zhang, Yorio, Jin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MB maintains neurosphere formation capacity of NPCs. (A–E) Detection of mRNA levels of stem cell, neural and glial markers in passage 1 (P1) NPCs. N = 3 in each group. (F) Serial passage analysis for neurosphere formation. N = 6 in each group. (G) Total cell number counting at the end of each passage. Note that at the beginning of each passage, only 1 × 10⁴ NPCs were seeded. (H) Western blot assay for Nestin, GFAP and DCX expression in P1 NPCs treated with MB for 6 days. Left, representative Western blot image. Right, statistical analysis for expression level of each protein. *p < 0.05; **p < 0.01; ***p < 0.001 compared with control group.