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. 2014 Oct 7;8:315. doi: 10.3389/fncel.2014.00315

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Copyright © 2014 Xie, Choudhury, Wang, Park, Liu, Yuan, Zhang, Yorio, Jin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MB does not impede committed neuronal differentiation. (A) Q-RTPCR analysis of the expression of differentiation markers in differentiated NPCs at 1 and 7 days after growth factors withdraw. N = 3 per group. ***p < 0.001 compared with day-1 differentiated NPCs. (B) Q-RTPCR for expression of differentiation markers in differentiated NPCs in the presence or absence of MB. N = 3 per group. (C) Map2 staining for differentiated NPCs in the presence or absence of MB. Upper, 50×; lower, 200×. (D) Total cell number counting and Map2⁺ cell number in five microscopy fields (50×). (E) Western blot assay for expression of differentiation markers in differentiated NPCs in the presence or absence of MB. This is a representative of two independent experiments.