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. 2014 Oct 7;8:315. doi: 10.3389/fncel.2014.00315

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Copyright © 2014 Xie, Choudhury, Wang, Park, Liu, Yuan, Zhang, Yorio, Jin and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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MB restrains NPC proliferation in vivo. (A) Detection of NPC proliferation with Nestin and BrdU staining. Left: representative confocal microscopy images (100×) of Nestin (green) and BrdU (red) at anterior and lateral part ventricular wall of SVZ in MB or vehicle treated rats. Arrows indicate Nestin⁺/BrdU⁺ cells. Right: quantification of Nestin⁺ cells and Nestin⁺/BrdU⁺ cells in the SVZ. (B) Representative confocal microscopy images (400×) of Nestin (green) and BrdU (red) at anterior and lateral part ventricular wall of SVZ. (C) Western blot assay for mTOR, p70S6K and cyclin D1 expression at SVZ in MB or vehicle-treated rats. (D) Statistical analysis for mTOR, p70S6K and cyclin D1 proteins at SVZ in MB or vehicle-treated rats. *p < 0.05; **p < 0.01 compared with control group.