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. 2014 Sep;184(9):2403–2419. doi: 10.1016/j.ajpath.2014.05.024

Figure 3.

Figure 3

Retinal abnormalities in Cyp27a1−/−Cyp46a1−/− mice. Representative SD-OCT fundus depth images (50-degree field of view) at the OPL in the Cyp27a1−/−Cyp46a1−/− animal (A and D) and sex-matched Cyp27a1+/+Cyp46a1+/+ littermate (G). Areas of pathology are outlined by ovals and circles. The areas examined in cross section (yellow) and the newly formed lesions (green). SD-OCT cross sections through the lesion area in the Cyp27a1−/−Cyp46a1−/− retina (B and E) and the manifestation of the Crb1rd8 mutation in the Cyp27a1+/+Cyp46a1+/+ retina (H). Approximately 80% of Cyp27a1−/−Cyp46a1−/− mice and matching Cyp27a1+/+Cyp46a1+/+ littermates are homozygous for Crb1rd8, approximately 15% are heterozygous, and approximately 5% lack the mutation. The Doppler flow (C, F, and I) of B, E, and H showing the RCA (F) and the separation of the retinal and choroidal vascular networks (C and I). The pattern of SD-OCT changes is similar in males and females and between animals with a different number of hyperreflective spots. Scale bars: 300 μm (A, D, and G); 100 μm (B, C, E, F, H, and I). Ch, choroid; GCL, ganglion cell layer; IPL, inner plexiform layer; mo, months; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium; SD-OCT, spectral-domain optical coherence tomography.