Skip to main content
. 2014 Sep;184(9):2403–2419. doi: 10.1016/j.ajpath.2014.05.024

Figure 4.

Figure 4

Different types of vascular abnormalities in the Cyp27a1−/−Cyp46a1−/− retina. Fluorescein angiography (FA) of Cyp27a1+/+Cyp46a1+/+ (A–C) and the vascular leakage (arrows of the same color indicate the same lesion) of Cyp27a1−/−Cyp46a1−/− mice (F–H, J–M, and O–R). SD-OCT fundus images (50-degree field of view) (D and I). TEM of the retinal cross section through the representative lesion (yellow arrow in F–I) with vascular leakage documenting a blood vessel in the RPE (yellow oval) and no membrane infoldings (blue arrow in E) (N). Representative FA images of other types of vascular abnormalities in Cyp27a1−/−Cyp46a1−/− mice (J–M and O–R); vascular nonperfusion (white ovals and circle) (J–M); arteriovenous shunts (fuchsia ovals) (J and O–Q); and vascular deformations (gray arrow) and focal defects (red circle) (R). Retinal analyses by elastase digests (S–U) show nondilated and dilated hypercellular blood vessels (green oval and blue circles, respectively, in T); membrane sheets with and without pigment (solid and dashed black circles, respectively, in T); intraretinal microvascular abnormality consisting of hypercellular condensed retinal vasculature (orange circle in U); and degenerated capillaries (magenta arrows in U; green arrows point to normal capillaries). Scale bars: 100 μm (A–C, F–H, J–M, and O–R); 2 μm (E and N); 50 μm (S–U). RPE, retinal pigment epithelium; SD-OCT, spectral-domain optical coherence tomography.