Figure 8.

Increased inflammatory response and oxidative stress in the Cyp27a1^−/−Cyp46a1^−/− retina. Representative stainings for the α_Mβ₂ integrin (CD11b) (A and B), complement factors C3/C3a and C9 (C–F), iso[4]levuglandin E₂ (M and N), and 7-ketocholesterol (7-keto; O and P). Corresponding images for a Cyp27a1^+/+Cyp46a1^+/+ mouse (G–L and Q–T). Control sections (Ctrl) were treated with the serum from nonimmunized animals. White arrows and rounded rectangles indicate increased staining compared with the Cyp27a1^+/+Cyp46a1^+/+ retinas. Immunostaining for 7-ketocholesterol likely represents antibody binding to both esterified and unesterified forms of the sterol, whereas the measurements in Figure 1 are of unesterified 7-ketocholesterol only. U: Histological quantifications. Scale bar = 100 μm (A–T). GCL, ganglion cell layer; INL, inner nuclear layer; IPL, inner plexiform layer; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigment epithelium.