. 2014 May 6;25(3):408–411. doi: 10.1007/s13337-014-0213-0

Table 1.

Results of sandwich ELISA (s-ELISA) and RT-PCR assays employed to detect PPRV antigen/nucleic acid in blood samples collected after twenty-first days post infection (dpi) to 397 dpi from experimentally infected cattle

Animal no	Mode of exposure to PPRV	Assays employed on different samples	Days post infection (dpi)
Animal no	Mode of exposure to PPRV	Assays employed on different samples	21	45	50	57	65	95	111	119	148	190	203	397
92	S/C inoculation	s-ELISA^a	++	++	+++	+ +	++	+	–	–	–	–	++	+++
92		RT-PCR^b	+	+	+	+	+	+	–	–	–	+	+	+
95		s-ELISA^a	++	++	+++	+++	+++	++	+	++	+ +	+	++	++
95		RT-PCR^b	+	+	+	+	+	+	+	+	+	+	+	+
190	Direct contact with infected goats	s-ELISA^a	+	+	+	+	++	+	++	+	+	+	++	+++
190		RT-PCR^b	+	+	+	+	+	+	+	+	+	+	+	+
185		s-ELISA^a	++	++	++	++	+	+	+	+	+ +	+	++	+++
185		RT-PCR^b	+	+	+	+	+	+	+	+	+	+	+	+
182		s-ELISA^a	–	+	+	+++	+	+	+	++	++	++	+++	+++
182		RT-PCR	+	+	+	+	+	+	+	+	+	+	+	+

^aSandwich ELISA with reactivity in terms of OD₄₉₆ value against each samples ranges mentioned in the parenthesis +++, strongly positive (0.76–1.4); ++, positive (0.186–0.67);+, Weakly positive (0.115 to 0.184); −, negative (0.089 to 0.096)

^bN gene-based RT-PCR assay for PPRV nucleic acid detection