Table 3. Steady-State Rates of Aminoacylation by Y59 IleRS Variantsa.
| kobs (s–1) | |
|---|---|
| wt IleRS | 0.86 ± 0.2 |
| Y59T | 0.074 ± 0.003 |
| Y59F | 0.024 ± 0.001 |
All substrate concentrations are verified to be saturating; therefore, kobs approaches kcat. The values represent the mean ± the SEM of at least three independent experiments.