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. 2014 Sep 10;53(39):6189–6198. doi: 10.1021/bi5007699

Table 3. Steady-State Rates of Aminoacylation by Y59 IleRS Variantsa.

  kobs (s–1)
wt IleRS 0.86 ± 0.2
Y59T 0.074 ± 0.003
Y59F 0.024 ± 0.001
a

All substrate concentrations are verified to be saturating; therefore, kobs approaches kcat. The values represent the mean ± the SEM of at least three independent experiments.