Schematic
and design of SR-BI/CD36 chimeric receptors. (A) Panel
of SR-BI/CD36 chimeras created by replacement of small regions of
SR-BI sequence with the corresponding CD36 sequence with a similar
secondary structure (avoiding sites of glycosylation and disulfide
bonds). Shown is a linear representation of SR-BI, with boxed gray
regions representing the N- and C-terminal transmembrane domains (TM),
black boxes representing all swapped regions (with residue ranges),
and hexagons marking residues that harbor N-linked glycosylations.
(B) SR-BI (GenBank accession number Q61009) and CD36 (GenBank accession number AAH72543) sequences
were aligned using ClustalW, and secondary structure prediction was
conducted using Jpred. Boxed regions represent sequences with similar
secondary structures. The symbols represent the following: asterisks
for fully conserved residues, colons for highly similar residues,
periods for weakly similar residues, dollar signs for Cys residues,
number signs for Asn residues of the N-linked glycosylation consensus
sequence, E for extended (β sheet), H for α helix, and
dashes for random coils.