Figure 3.

HDL binding and selective uptake of HDL-COE in cells expressing wild-type SR-BI and SR-BI/CD36 chimeric receptors. COS-7 cells transiently expressing wild-type SR-BI or SR-BI/CD36 chimeric receptors were incubated at 37 °C for 1.5 h with [¹²⁵I]DLT/[³H]COE-labeled HDL (10 μg of HDL protein/mL), after which cells were processed. In parallel, cells were plated in separate wells to assess cell surface expression by flow cytometry. Cell-associated HDL (A) and selective uptake of HDL-COE (B), both normalized to cell surface expression, are shown. Data represent means ± SD of at least three independent experiments, each performed in triplicate. As determined by one-way ANOVA, *p = 0.01–0.05, **p < 0.005, and ***p < 0.001.