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. Author manuscript; available in PMC: 2014 Oct 7.
Published in final edited form as: Cancer Res. 2009 Feb 24;69(5):1836–1843. doi: 10.1158/0008-5472.CAN-08-4103

Figure 6. Differential effects of PIs on UPR and HIF-1α protein levels in different cancer cells.

Figure 6

A. Differential effects of PIs on eIF2α phosphorylation. PC3, DU145 and 253JB-V cells were incubated with 100nM bortezomib, 100nM NPI-0052, or 10μM thapsigargin for 4h. Phosphorylated eIF2α(Ser52) and total eIF2α levels were measured by immunoblotting. The numbers located below each lane corresponded to the levels of phospho-eIF2α which were determined by densitometry analysis and adjusted to total eIF2α protein levels. The phosphorylation of eIF2α in the untreated group was arbitrarily set at the value of 1. B. Differential effects of PIs on protein synthesis. PC3, DU145 and 253JB-V cells were treated as above for 4h and protein synthesis was measured by [L-4,5-3H]Leucine incorporation. Data shown are mean±S.E., *P<0.001, compared to control. C. Differential effects of PIs on HIF-1α. PC3, DU145 and 253JB-V cells were incubated with 100nM bortezomib or 100nM NPI-0052 for 12h under hypoxic conditions and HIF-1α levels were measured by immunoblotting.